Protective Effects of a Hydrogen-Rich Preservation Solution in a Canine Lung Transplantation Model

BackgroundMolecular hydrogen (H2) has protective effects against ischemia-reperfusion injury in various organs. Because they are easier to transport and safer use than inhaled H2, H2-rich solutions suitable for organ preservation. In this study, we examined the of an solution lung preservation a canine left transplantation (LTx) model.MethodsTen beagles underwent orthotopic LTx after 23 hours cold ischemia followed by reperfusion 4 hours. Forty-five minutes reperfusion, right main pulmonary artery was clamped evaluate function implanted graft. The were divided into two groups: control group (n = 5), H2 5). group, donor lungs flushed immersed during at 4°C using ET-Kyoto solution, these solution. Physiologic assessments performed reperfusion. After wet-to-dry ratios determined, histology examinations performed.ResultsSignificantly higher partial pressure arterial oxygen significantly lower carbon dioxide observed (P .045 P < .001, respectively). ratio .032). Moreover, examination, less fewer apoptotic cells .001 respectively).ConclusionsOur results demonstrated that attenuated model. Molecular Ten performed. Significantly Our